atgenexpress developmental time-line data set  (Schmid GmbH)

Journal: Plant Physiology

Article Title: A Deficiency in the Flavoprotein of Arabidopsis Mitochondrial Complex II Results in Elevated Photosynthesis and Better Growth in Nitrogen-Limiting Conditions ^{1 [W] [OA]}

doi: 10.1104/pp.111.183939

Figure Lengend Snippet: SDH genes are highly expressed in guard cells. A, GUS expression in guard cells from Arabidopsis transgenic plants carrying a SDH1-1 promoter fusion. Histochemical localization of GUS activity is shown for leaves of 2-week-old seedlings grown under axenic conditions. The SDH1-1 promoter (788 bp) drives high expression in guard cells. Bar = 25 μm. B, Expression of complex II genes in mesophyll and guard cells. Data used to generate the digital northern blot were obtained using the Arabidopsis eFP browser at http://bar.utoronto.ca (Winter et al., 2007). For each gene, expression was normalized with respect to the median value across essentially all wild-type samples in the AtGenExpress tissue data set (Schmid et al., 2005).

Article Snippet: For each gene, expression was normalized with respect to the median value across essentially all wild-type samples in the AtGenExpress tissue data set ( Schmid et al., 2005 ).

Techniques: Expressing, Transgenic Assay, Activity Assay, Northern Blot, Gene Expression

Journal:

Article Title: Requirement of B2-Type Cyclin-Dependent Kinases for Meristem Integrity in Arabidopsis thaliana [W] [OA]

doi: 10.1105/tpc.107.054676

Figure Lengend Snippet: Relative Expression Levels of CDKA;1, CDKB2;1, and CDKB2;2. (A) The CDKB2s were highly expressed in the apex, whereas CDKA;1 was expressed at similar levels across all tissues. Expression levels were normalized to the per-gene average across all samples. (B) CDKB2 expression was reduced in stm and wus mutants, which have impaired meristem function, while their transcript levels were increased in clv3 mutants with enlarged meristems. Expression levels were normalized to the wild-type control. (C) The CDKB2s showed a peak in expression level at the G2/M transition, while CDKA;1 expression did not change in response to the cell cycle phase. Transcript abundance was normalized to the expression level at the time of cell cycle block release. Microarray data were extracted from the AtGenExpress compendium (Menges et al., 2003; Schmid et al., 2005) and represent biological triplicates in (A) or biological duplicates in (B) and (C).

Article Snippet: In the AtGenExpress developmental data set ( Schmid et al., 2005 ), expression of these genes was highest in samples corresponding to the shoot apex ( ) but was strongly reduced in shoot apices of 3-d-old wus and stm mutant seedlings, which fail to maintain a proper SAM ( ).

Techniques: Expressing, Control, Blocking Assay, Microarray